An established and comprehensive range of molecular genetic services from assay design to data analysis including: DNA extraction, microarray services, qPCR and next generation sequencing.
We offer a fully customisable service utilising Invader and InvaderPlus chemistries for biomarker discovery, assay development, assay validation and companion diagnostic development.
- Invader and InvaderPlus assays are suitable for a wide range of applications including genotyping, copy number variation and detection of InDels
- Proprietary, cost effective isothermal, DNA-probe based system
- Scalable and automatable
- Currently used in multiple FDA approved and CE marked products
- Works with a wide range of tissue types including FFPE
- Custom assays available
In the first reaction, two oligonucleotides, a probe and an Invader oligo, anneal to a specific DNA target sequence to generate a one-base overlapping structure if the desired sequence is present. Each time an intact probe molecule binds to the specific target in the presence of the Invader oligo, the overlapping substrate is formed and cleavage occurs. The number of flaps released is relative to the amount of target in the sample, allowing for quantitative detection of genes, chromosomes or infectious agents.
Secondary, simultaneous reaction
Cleaved flaps from the primary Invader reaction combine with a fluorescence resonance energy transfer (FRET) probe in a secondary, simultaneous overlapping cleavage reaction, generating a fluorescent signal. The combination of two different flap sequences, FRET oligos, and fluorophores allows for single-well biplex reactions to occur.
Each released 5’ flap from the primary reaction continually binds and releases from the FRET cassettes, enabling the secondary reaction to further amplify the target-specific signal. The two simultaneous reactions typically produce a 1-10 million-fold signal amplification during a 1-4 hour reaction.
The use of discriminatory primary probes (wild-type and mutant), and two corresponding FRET cassettes, each with a distinct fluorophore, produces target-specific signals that allow for distinguishing between wild-type, mutant and heterozygous-target sequences.
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Affymetrix microarray services
Tepnel is an Authorised Affymetrix Service Provider. The authorised Affymetrix service provider status demonstrates a level of commitment to customer satisfaction through the processes and quality measures that generate high-quality data. Tepnel is able to offer a broad range of Affymetrix services including:
Full range of assays and microarray solutions for 3′ IVT Expression Analysis, Whole-Transcript Expression Analysis, miRNA Analysis. All assays can be carried out using standard or low input amplification kits which allow the use of small RNA inputs as well as the use of degraded FFPE material.
DMET Plus Solution. The broadest coverage, single pharmacogenetic assay that enables the most rapid genetic analysis of the involvement of metabolic pathways in drug metabolism.
Tepnel is approved to run the OncoScan FFPE Assay Kit, which allows access to an oncology specific panel for high-quality copy number and genotype data with whole-genome coverage and high resolution in cancer genes for use with challenging FFPE samples. Based on Affymetrix’ unique Molecular Inversion Probe (MIP) technology, OncoScan is capable of analysing small amounts of highly degraded DNA from FFPE samples quickly and affordably, providing a significant step forward in solid tumor cancer analysis.
Illumina microarray services
Tepnel is a certified CSPro Service Provider offering the entire Illumina range of microarrays. These cover a wide range of human and non-human applications:
- SNP genotyping and CNV analysis
- Methylation profiling
- Human gene expression analysis
- Formalin Fixed Paraffin Embedded Tissue (FFPE). Both SNP genotyping and methylation analysis can be carried out using FFPE tissue
- Custom microarray design service available
Via our high throughput qPCR systems we are able to offer a complete range of qPCR solutions including gene expression, SNP genotyping, InDels, copy number variation and residual DNA analysis using both custom and catalogue assays.
Sanger Sequencing and fragment analysis
Sanger sequencing and fragment analysis using the ABI3130xl (including UGT1A1 *1/28/36/37 assay).
Ion Torrent PGM Next Generation Sequencing
Tepnel is a certified Ion Torrent service provider. With the Ion Torrent Personal Genome Machine (PGM), researchers can detect variants by targeted gene sequencing in cancer and genetic disorders, and microbiologists can easily type a bacteria or virus, or characterise novel microbes. Equipped with short and long read sequencing, the Ion PGM System is suitable for a wide range of applications including:
Amplicon sequencing using Ion AmpliSeq
Ion AmpliSeq technology delivers simple and fast library construction for targeted sequencing of specific human genes or genomic regions. Based on ultrahigh-multiplex PCR, Ion AmpliSeq technology can be used for the identification of SNPs, CNVs, gene fusions, and InDels.
AmpliSeq technology can be used to interrogate DNA samples to identify both somatic and germline mutations and CNV alterations. RNA panels enable scientists to choose from over 20,000 targeted genes to survey gene expression fold changes. In addition to custom panels, there are several Ion AmpliSeq ready-to-use panels available.
The Ion Torrent PGM offers a full solution for small genome sequencing and bacterial/viral typing:
- De novo sequencing of microorganisms
- Bacterial 16s typing using the Ion 16s Metagenomics kit
- Viral typing
The Luminex 200 System is capable of multiplexing up to 100 different tests in a single reaction volume. Assays can be both custom and catalogue including assays from both Luminex as well as third party vendors:
- Gene expression
- Genotyping, e.g. Cytochrome P450 2D6 and Cytochrome P450 2C19
- HLA typing assays