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DNA / RNA Extraction

The isolation and purification of DNA or RNA is the basic building block and an essential requirement for all downstream genomic analyses. For more than 16 years, our proprietary Nucleon extraction chemistry has provided a means to extract high quality DNA. This is the earliest step in many research or diagnostic processes used to understand disease and to diagnose genetic disorders. Our DNA extraction solution has been designed specifically for the efficient recovery of high yields of very high molecular weight, pure DNA suitable for long term biobanking.

About Nucleon Chemistry

The Nucleon extraction chemistry rapidly and efficiently extracts high yields of pure, high molecular weight DNA. Nucleon is compatible for use with whole blood and other sample types such as tissue and saliva. Benefits include:

Efficiency – The DNA is not bound to a solid surface at any point in the process. This avoids exposing the DNA to shearing forces and promotes the recovery of high yields of highly intact DNA.

Purity – The recovered DNA is free from impurities that might affect downstream applications and consistently provides A260/A280 ratios of ≥ 1.80.

Principle – Following cell lysis, deproteinisation with sodium perchlorate and a single partition against chloroform, the Nucleon proprietary resin is added. This resin covalently binds proteins and subsequently forms a semi-solid stratum during partitioning that traps proteinaceous material at the interface and in the organic phase. This facilitates removal of the aqueous phase, ensuring excellent recovery of high quality DNA. The Nucleon DNA extraction chemistry is not based on spin columns or magnetic beads, consequently scaling up to large input sample volumes/weights is simple and cost effective.

Performance data:

High yield (370-800μg/10ml blood)

High purity (A260/A280 > 1.8)

High MWt DNA (>50kb)

Economical large volume sample extraction

Fully scalable (0.3mL to 50mL blood and 5mg to 500mg tissue)

Suitable for recovery of usable yields of DNA from aged blood

DNA extraction services

We provide a rapid and affordable DNA extraction service which generates high quality and high yield DNA using our proprietary Nucleon DNA extraction chemistry. Using this and other validated procedures we are able to extract DNA from a broad range of biological matrices including:

Rapid DNA extraction services

As part of our services portfolio we also offer a rapid turnaround DNA extraction service. We can also extract from low volume blood samples using a commercially available magnetic bead-based chemistry. The resultant DNA is suitable for use in a range of downstream applications including SNP genotyping on Illumina and Affymetrix platforms and Taqman/qPCR/Invader SNP assays, sequencing and copy number variation.

RNA extraction services

We have proven experience in RNA isolation from a number of different sample types including:

We also offer a number of associated QC options including agarose gel electrophoresis and Bioanalyzer analysis. Our RNA extraction services also include a rapid extraction service.

miRNA extraction services

In addition to total RNA isolation we also offer a miRNA isolation service. We are able to extract miRNA from a number of different matrices including:

We also offer a number of associated QC options including agarose gel electrophoresis and bioanalyzer analysis. Our RNA extraction services also include a rapid extraction service.

Sections in Research Use Only Services

All Research Use Only Services

Additional Resources

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